RESUMEN
A large number of Endotrypanum stocks (representing an heterogeneous population of strains) have been screened against a panel of monoclonal antibodies (MAbs) derived for selected species of Endotrypanum or Leishmania, to see wether this approach could be used to group/differentiate further among these parasites. Using different immunological assay systems, MAbs considered specific for the genus Endotrypanum (E-24, CXXX-3G5-F12) or strain M6159 of E. schaudinni (E-2, CXIV-3C7-F5) reacted variably according to the test used but in the ELISA or immunoflurescence assay both reacted with all the strains tested. Analysis using these MAbs showed antigenic diversity occuring among the Endotrypanum strains, but no qualitative or quantitative reactivity pattern could be consistently related toparasite origin (i.e., host species involved) or geographic area of isolation. Western blot analysis of the parasites showed that these MAbs recognized multiple components. Differences existed either in the epitope density or molecular forms associated with the antigenic determinants and therefore allowed the assignment of the strains to specific antigenic groups. Using immunoflurescence or ELISA assay, clone E-24 produced reaction with L. equatorensis (which is a parasite of sloth and rodent), but not with other trypanosomatids examined. Interestingly, the latter parasite and the Endotrypanum strains cross-reacted with a number of MAbs that were produced against members of the L. major-L. tropica complex.
Asunto(s)
Animales , Anticuerpos Monoclonales , Trypanosomatina , Ensayo de Inmunoadsorción Enzimática/estadística & datos numéricosRESUMEN
As part of our ongoing program, searching for the vectors and reservoirs of Leishmaniaparasites, we conducted a survey of the sandfly fauna along the BR-319 (Manaus-Porto Velho) highway. Fifty seven species of sandflies were captured; 44 in the genus Lutzomyia and 13 Psychodopygus.Of the collecting methods utilized, CDC traps set 6m above the forest floor proved to be the most effective, and the most frequently captured species were L. antunesi, P. chagasiand P. ayrozai.We also present the geographical distribution of the different species collected.
Como parte do programa do laboratorio de Parasitología do INPA, na procura dos vetores e reservatórios da Leishmaniose, foi realizado durante o periodo de 1978 até 1981, um levantamento da fauna flebotomínica, em oito localidades da Rodovia BR-319 (Manaus-Humaita). Capturamos 57 espécies: 44 do gênero Lutzomyiae 13 de Psychodopygus. Os métodos de coleta usados foram: capturas nos troncos das árvores, isca humana e armadilhas luminosas, o método mais eficiente foi o da armadilha luminosa colocada a 6 metros de altura na floresta. As espécies mais comuns foram L. antunesi, P. chagasie P. ayrozai.Dados sobre a distribuição geográfica das espécies coletadas e a distribuição por coletas são também assinalados.